Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts
Shinkafi Abubakar Liman,
Yarima Mohammaed Bello,
Deeni Yusuf Yahaya,
Danjuma Lawal
Issue:
Volume 8, Issue 2, June 2023
Pages:
30-36
Received:
22 July 2022
Accepted:
20 December 2022
Published:
22 May 2023
Abstract: M. indica and B. dalzeilii are common medicinal plants grown naturally in most parts of tropical and subtropical regions of Northern Nigeria. They are used traditionally in the treatment of diarrhea in children especially at teething age. This study was conducted to detect, isolate and identify E. coli O157:H7 from diarrheal stools of children and evaluate antibacterial activities of M. indica and B. dalzielii extracts against the test bacteria. Total number of one hundred (100) samples were collected from Children Clinic of General Hospital Gusau, Zamfara State Nigeria using standard microbiological procedure. E. coli O157:H7 is an important pathogens of human, implicated as one of the important causative agents of diarrhea in children especially at teething ages. The test organism was detected, isolated and identified using Cultural Growth Characteristics, Gram reactions, Serology, Biochemical tests and Molecular screenings. The sample plants extracts were obtained using Soxhlex Apparatus with Methanol as solvent. Phytochemical screening of the plants extracts revealed the presence of Alkaloids, Flavoids, Tannins and Saponnins. Antibacterial activities of the aqueous methanolic extracts of the test plants against E. coli O157:H7 was evaluated using Sensitivity Tests, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC). The most active phytochemical compounds was obtained by fractionation using Chromatography. Chemical structure of the most active phytochemical was elucidated using Nuclear Magnetic Resonance Spectroscopy. Confirmation of antibacterial activities of the test plants, lend credence to the traditional used of those plants in the treatment of diarrhea in children especially at teething ages. It is therefore recommended that, M. indica and B. dalzeilii can be further purified to yield templates for synthesis of Orthodox drugs for the treatment of diarrhea in children.
Abstract: M. indica and B. dalzeilii are common medicinal plants grown naturally in most parts of tropical and subtropical regions of Northern Nigeria. They are used traditionally in the treatment of diarrhea in children especially at teething age. This study was conducted to detect, isolate and identify E. coli O157:H7 from diarrheal stools of children and ...
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Microbial Lipases: Versatile Enzymes Which Hold Immense Potential in Industrial Application
Issue:
Volume 8, Issue 2, June 2023
Pages:
37-42
Received:
10 April 2023
Accepted:
10 June 2023
Published:
21 June 2023
Abstract: Lipases abundant in nature have a wide application in several industrial sectors. They can be isolated from a variety of sources but lipases isolated from microbes have huge demand due to the easy availability, cost efficiency, greater yield, simple genetic manipulation and higher thermostability. The lipase market is valued at US$ 690 Mn in 2021. It is set to grow at 7.8% CAGR through 2026. The global microbial lipase market size is expected to reach US$ 915.9 Bn by the end of 2029. Lipases are known to play important role in esterification, alcoholysis and hydrolysis. Extracellular enzymes produced from microbes are in great demand due to its wide application in industry related to improving the manufactured products. Lipases have become important in applied and industrial microbiology as also in enzyme engineering sector. Thermostable lipases are in demand as it can carry out chemical reactions at higher temperature without alteration of the structure and activity of the enzyme as also faster reaction and increased solubility. They act as biological catalysts meeting the requirement in several industries like dairy, food, brewery, pharmaceuticals and medicine, detergent, leather and biodiesel. This review is focused on the different application of lipases in industry, the purification methods of lipases and the methods for their assay measurement.
Abstract: Lipases abundant in nature have a wide application in several industrial sectors. They can be isolated from a variety of sources but lipases isolated from microbes have huge demand due to the easy availability, cost efficiency, greater yield, simple genetic manipulation and higher thermostability. The lipase market is valued at US$ 690 Mn in 2021. ...
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Emergence of West Nile Virus in Ivory Coast
Kamo Irie Lou Bohila Emilie,
Sylla Yahaya,
Diaha-Kouame Claude Aimee,
Diane Kouao,
Adjogoua Edgard Valery,
Adje Kadio Herve,
Dosso Mireille
Issue:
Volume 8, Issue 2, June 2023
Pages:
43-47
Received:
9 June 2023
Accepted:
28 June 2023
Published:
6 July 2023
Abstract: West Nile fever is a neglected endemic pathology in West Africa. It is caused by a flavivirus. The objective of this work is to search from the national surveillance of yellow fever and Dengue viruses, the West Nile virus for the year 2019. Sera from samples diagnosed negative for yellow fever and dengue fever were used to detect specific anti-yellow fever virus IgM antibodies by immunocapture using the ELISA (Enzyme-Linked ImmunoSorbent Assay) technique. The real-time molecular method (RT-PCR) was also used to search for the viral genome through the envelope gene (E). Serological results revealed serum IgM antibodies against West Nile virus. We have 03 positive cases/132 cases studied with 3.17% positive in women and 1.67% of cases in men. It affected children aged 15 to 17 (0.76%) and adults over 40 (1.53%). Only the districts of Abidjan were exposed. Regarding the PCR test, all samples for the viral genome were negative. This study indicates the presence of antibodies against the circulating West Nile virus in Abidjan. These West Nile cases detected in the Abidjan health district have highlighted the reality of West Nile.
Abstract: West Nile fever is a neglected endemic pathology in West Africa. It is caused by a flavivirus. The objective of this work is to search from the national surveillance of yellow fever and Dengue viruses, the West Nile virus for the year 2019. Sera from samples diagnosed negative for yellow fever and dengue fever were used to detect specific anti-yell...
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